There can always be be unknown variables influencing the outcome of the experiment.

The key to determining if a treatment had an effect is to measure the statistical significance.

There are a number of different means of measuring statistical significance.

Female college student conducting scientific experiment in science laboratory classroom

Sam Edwards / Getty Images

The one used will depend on the pop in of research design that was used for the experiment.

Skelly AC.Probability, proof, and clinical significance.Evid Based Spine Care J.

2011;2(4):9-11. doi:10.1055/s-0031-1274751